Asymmetric siRNA: New Strategy to Improve Specificity and Reduce Off-Target Gene Expression
نویسندگان
چکیده
منابع مشابه
siRNA Target Finding Strategy
Machine learning algorithm Based on a large number of experimental data of RNAi and advanced data mining technology, GenScript now develops a newest machine learning algorithm to predict siRNA. Compared with our first generation design tool, the accuracy of new algorithm, especially for the design of shRNA, has greatly enhanced by 90%. The siRNA design algorithm developed by GenScript, along wi...
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MOTIVATION Designing highly effective short interfering RNA (siRNA) sequences with maximum target-specificity for mammalian RNA interference (RNAi) is one of the hottest topics in molecular biology. The relationship between siRNA sequences and RNAi activity has been studied extensively to establish rules for selecting highly effective sequences. However, there is a pressing need to compute siRN...
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Artificially synthesized short interfering RNAs (siRNAs) are widely used in functional genomics to knock down specific target genes. One ongoing challenge is to guarantee that the siRNA does not elicit off-target effects. Initial reports suggested that siRNAs were highly sequence-specific; however, subsequent data indicates that this is not necessarily the case. It is still uncertain what level...
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The downregulation of many mRNAs has been observed through bioinformatic analysis of microarray results following transfection of short interfering RNAs (siRNAs). Many of these mRNA changes are due to the interaction of the siRNA guide strand with partially complementary sites and thus are considered "off-target" effects. To examine the mRNA:siRNA interactions important for off-target effects, ...
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Smith-Waterman alignment algorithm is favored in search for siRNA off-target instead of the BLAST algorithm, because BLAST tends to overlook some significant homologous sequences, especially when they are short (21 nt~27 nt). Smith-Waterman algorithm, however, suffers from its own shortcomings, especially its inefficiency in searching through a large sequence database. This paper presents a two...
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ژورنال
عنوان ژورنال: Human Gene Therapy
سال: 2012
ISSN: 1043-0342,1557-7422
DOI: 10.1089/hum.2011.145